ABSTRACT
Glioblastoma multiforme (GBM) is the most common primary intracranial tumor in adults and has poor prognosis. The GBM-specific tumor microenvironment (TME) plays a crucial role in tumor progression, immune escape, local invasion, and metastasis of GBM. Here, we demonstrate that hypoxia, reactive oxygen species (ROS), and differential concentration of glucose influence the expression of cytokines and chemokines, such as IL-6, IL-8, and IP-10, in human glial cell lines. Treatment with cobalt chloride (CoCl₂) and hydrogen peroxide (H₂O₂) significantly increased the expression levels of IL-6, IL-8, and IP-10 in a dose-dependent manner in CRT-MG and U251-MG astroglioma cells, but not in microglia cells. However, we found strikingly different patterns of expression of cytokines and chemokines between H₂O₂-treated CRT-MG cells cultured in low- and high-glucose medium. These results suggest that astroglioma and microglia cells exhibit distinct patterns of cytokine and chemokine expression in response to CoCl₂ and H₂O₂ treatment, and different concentrations of glucose influence this expression under either hypoxic or oxidant-enriched conditions.
Subject(s)
Adult , Humans , Hypoxia , Astrocytoma , Cell Line , Chemokines , Cobalt , Cytokines , Glioblastoma , Glucose , Hydrogen Peroxide , Hydrogen , Interleukin-6 , Interleukin-8 , Microglia , Neoplasm Metastasis , Neuroglia , Prognosis , Reactive Oxygen Species , Tumor Microenvironment , United NationsABSTRACT
PURPOSE: To report a case of presumed intraocular natural killer/T-cell lymphoma (NKTL). CASE SUMMARY: A 61-year-old male presented with visual disturbance of the left eye for duration of 3 years. He had been treated with systemic chemotherapy and radiotherapy for nasal NKTL 1 year prior. Inflammatory reaction in the anterior chamber and vitreous opacity were observed in the left eye. The patient was diagnosed with uveitis in the left eye at a local clinic 3 years prior to visiting us. Because the patient did not respond to anti-inflammatory therapy, we performed diagnostic and therapeutic vitrectomy. Intraoperatively, vitreous opacity was thin sheet like in appearance. The vitreous specimen contained few lymphoid cells and was positive for Epstein Barr virus-encoded RNA (EBER). Systemic workups showed no metastasis to other organs. The patient was treated with systemic methotrexate chemotherapy and intravitreal methotrexate injected 3 times (once per week). During the 12-month follow-up period after the last intravitreal injection, the recurrence of lymphoma and related uveitis was not observed.
Subject(s)
Humans , Male , Middle Aged , Anterior Chamber , Eye , Follow-Up Studies , Intravitreal Injections , Lymphocytes , Lymphoma , Methotrexate , Neoplasm Metastasis , Recurrence , RNA , Uveitis , VitrectomyABSTRACT
Kaposi's sarcoma (KS) is an unusual multifocal neoplasm of vascular endothelial cell origin. The trunk, arms, head, and neck are the most common sites. It is common in men and has four distinct variants: classic, Africa-endemic, immunosuppressive drug-associated, and acquired immunodeficiency syndrome-associated KS. KS appears to develop immunosuppressed patients, but is uncommon in patients on dialysis. A 79-year-old man on hemodialysis for 2 months presented with pruritus over the entire body and multiple, discrete, variable-sized, dark blue papulonodules (papuloplaques, maculopapules) on the left arm and shoulder. A biopsy specimen form the left arm showed spindle cells with slit-like spaces and extravasated red blood cells. The specimen was positive for CD 34 antigen, and human herpesvirus 8 was detected. We report a case of KS that occurred in a 79-year-old patient on hemodialysis.
Subject(s)
Aged , Humans , Male , Arm , Biopsy , Dialysis , Endothelial Cells , Erythrocytes , Head , Herpesvirus 8, Human , Neck , Pruritus , Renal Dialysis , Sarcoma, Kaposi , ShoulderABSTRACT
Actinomyces normally colonizes the mouth, colon, and vagina. Although disruption of mucosa may lead to infection at virtually any site, central nervous system actinomycosis is rare. A 45-year-old man presented with seizure and magnetic resonance imaging showed brain abscess. He was diagnosed with actinomycotic and streptococcal infection of brain by histologic and microbiologic examination. After stereotactic aspiration and biopsy, he was treated successfully by prolonged antibiotic therapy using intravenous penicillin-G and oral amoxicillin.
Subject(s)
Humans , Middle Aged , Actinomyces , Actinomycosis , Amoxicillin , Biopsy , Brain , Brain Abscess , Central Nervous System , Colon , Magnetic Resonance Imaging , Mouth , Mucous Membrane , Seizures , Streptococcal Infections , VaginaABSTRACT
Actinomyces normally colonizes the mouth, colon, and vagina. Although disruption of mucosa may lead to infection at virtually any site, central nervous system actinomycosis is rare. A 45-year-old man presented with seizure and magnetic resonance imaging showed brain abscess. He was diagnosed with actinomycotic and streptococcal infection of brain by histologic and microbiologic examination. After stereotactic aspiration and biopsy, he was treated successfully by prolonged antibiotic therapy using intravenous penicillin-G and oral amoxicillin.
Subject(s)
Humans , Middle Aged , Actinomyces , Actinomycosis , Amoxicillin , Biopsy , Brain , Brain Abscess , Central Nervous System , Colon , Magnetic Resonance Imaging , Mouth , Mucous Membrane , Seizures , Streptococcal Infections , VaginaABSTRACT
PURPOSE: The aim of this study was to investigate the clinical usefulness of upper gastrointestinal (GI) endoscopy in children with Henoch-Schonlein purpura (HSP). METHODS: We retrospectively analyzed the clinical, endoscopic, and histopathologic records of children with HSP who had been admitted to the Department of Pediatrics of Gil Hospital and underwent upper GI endoscopy between January 2002 and June 2009. Patients were classified into the following two groups for statistical analysis: duodenal involvement (+) and duodenal involvement (-). RESULTS: Fifty-one children with HSP underwent upper GI endoscopy; the mean age was 7.2+/-2.9 years. The upper GI endoscopy showed abnormalities of the duodenum in 38 cases (74.5%), 22 of which had duodenal ulcers. Among the biopsy specimens obtained from the duodenum of 37 cases, 13 cases (35.1%) had leukocytoclastic vasculitis, neutrophil debri, and/or extravasation of RBCs. Steroid use was more frequent in the duodenal involvement (+) group (86.8%) than the duodenal involvement (-) group (53.8%; p=0.02). The mean length of hospitalization was 13.9+/-8.43 days in the duodenal involvement (+) group and 8.1+/-4.62 days in the duodenal involvement (-) group (p=0.003). The recurrence rate was significantly higher in the duodenal involvement (-) group than the duodenal involvement (+) group (p=0.027), whereas none of the other study parameters, such as the age of onset, renal involvement, and steroid use, led to significantly higher or lower recurrence rates. CONCLUSION: These results suggest that duodenal involvement can influence the clinical course and prognosis of HSP in children.
Subject(s)
Child , Humans , Age of Onset , Biopsy , Duodenal Ulcer , Duodenum , Endoscopy , Endoscopy, Gastrointestinal , Hospitalization , Neutrophils , Pediatrics , Prognosis , IgA Vasculitis , Recurrence , Retrospective Studies , Vasculitis , Vasculitis, Leukocytoclastic, CutaneousABSTRACT
About 1% of malignant melanoma is found in the head and neck region and among them, about 2-16% is presented with palpable regional metastatic melanoma with unknown primary lesion (MUP). We have experienced two cases of MUP which were diagnosed after excisional biopsy. MUP has better prognosis than that with known primary lesion. To get good prognosis, the initial treatment of MUP should be regional lymphadenectomy, indicating the importance of an accurate diagnosis. Although the radiologic finding for malignant melanoma may offer no specific findings, physicians should be aware of the possibility of melanoma in neck mass patients. We present two cases of MUP with a literature review.
Subject(s)
Humans , Biopsy , Head , Lymph Node Excision , Melanoma , Neck , Neoplasm Metastasis , PrognosisABSTRACT
Primary adenosquamous carcinoma of the ampulla of Vater is extremely rare. We report two cases of adenosquamous carcinoma of the ampulla of Vater in a 48-year-old female and an 80-year-old female patient. The gross appearance was a polypoid mass in both cases. Histologically, the majority of the two tumors was composed of squamous cell carcinoma with some intermingled glandular adenocarcinoma components in both cases. Squamous cell carcinomas showed an abrupt transition from the normal glandular epithelium or glandular adenocarcinoma. Although the two cases revealed duodenal wall invasion and lymph node metastasis was found in case 2, both patients are still alive at 19 and 46 months after surgery, respectively.
Subject(s)
Aged, 80 and over , Female , Humans , Middle Aged , Adenocarcinoma , Ampulla of Vater , Carcinoma, Adenosquamous , Carcinoma, Squamous Cell , Epithelium , Lymph Nodes , Neoplasm MetastasisABSTRACT
This study aims to investigate visual tracking task of Koreans to the various visual stimuli by measuring the horizontal saccadic reaction times. The measuring system consists of eye monitor using infra-red reflection technique, oscillographic recorder and forehead-chin fixation devices. The visual stimuli generated by target function generator displayed simple-step, pulsestep and double-step stimulus patterns. The results revealed that the sacoadic reaction time showed no significant changes between the size of target displacement within horizontal 20 degrees. Furthermore, no significant changes between the particular direction of stimulus could be found. The nominal value of reaction time was 259 +/- 25 msec.
Subject(s)
Reaction TimeABSTRACT
This study aims to ascertain the human saccadic visual tracking characteristics by measuring the eye movement to the double-step stimuli. Responses to simple step and double-step target motions were measured where the target mode and stimulus durations were randomized. To measure the eye movement the Photo-electric Eye Monitor was constructed based on limbus tracking. The results indicate that observers represented two kind of responses (A-or B-type) depending upon the stimulus duration. The percentage of occurrances increased as the stimulus duration increased from 50 to 200 msec. When the subject responded to both target motions, the reaction time of the second saccade was shorter than that of the first. The successive visual information is to change continuously the reaction time or cancel the initial saccade. Our results revealed that parallel data processing characteristics of the visual tracking systems and the saccadic decision-makings are influenced by the temporal relationships.
Subject(s)
Humans , Eye Movements , Reaction Time , SaccadesABSTRACT
The saccadic reaction times to the double-step stimuli were studied in three human subjects to ascertain the saccadic visual tracking characteristics. Responses to simple step and double-step target motions were measured where the target mode and stimulus durations were randomized. To measure the eye movement the Photo-electric Eye Monitor was constructed based on limbus tracking. The results indicate that observers represented two kind of responses (A-or B-type) depending upon the stimulus duration. The percentage of occurrances increased as the stimulus duration increased from 50 to 200 msec. When the subject responded to both target motions, the reaction time of the second saccade was shorter than that of the first. The successive visual information is to change continuously the reaction time or cancel the initial saccade. Our results revealed that parallel data processing characteristics of the visual tracking systems and the saccadic decision-makings are influenced by the temporal relationships.
Subject(s)
Adult , Humans , Male , Eye Movements , Motion Perception , Reaction Time , Saccades , Visual PerceptionABSTRACT
A new method is described which uses photo-electric matrix method to measure two-dimensional eye movements. This system is composed of the detector unit (monitor), matrix and correction circuits and stimulus generator. A light and small detector unit attached to the modified trial frame transduces positional informations and can be achieved head-mounting. The instrument operates in the infrared, so that it does not interfere with normal vision, and over a two-dimensional visual field to +/-15 degrees. A system for generating a fixed target whose velocity, displacement, form, intensity, and other characteristics can be varied systematically also is described. The operating procedures are described and operating records are shown. Electrical and optical modifications of the present system will make possibIe the measurement of more smaller movement of the eye. Extensions and improvement of the technique of measurement are discussed briefly.
Subject(s)
Eye Movements , Visual FieldsABSTRACT
The purpose of this review is to examine the ways in which eye position and eye movements are measured in order to justify the choice of method for the study concerning the eye movement control systems. The oculomotor control systems were reviewed previously as a viewpoint of measuring objects. Because of the different aspects of eye movement which have been considered, various techniques have been developed-each having its own characteristics of range, sensitivity, bandwidth, stability and ease of application. This study is directed at discussing the pros and cons inherent in a basic method of measurement and to indicate the interesting historical development of oculography as new technologies were employed. Various methods are summarized and photo-electronic method(Limbus tracking technique) was chosen which is most available for the future experimentation.